SNU-324
| KCLB NO |
00324.1
|
|---|---|
| Name |
SNU-324
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
pancreas
|
|---|---|
| Species |
human, asian male, 50 years old
|
| Abnormal genes |
microsatellite instability
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer and floating
|
| Histopathology |
pancreatic carcinoma
|
| Depositor |
Jae-Gahb Park, M.D
|
| Option |
No Antibiotics
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''''''''''''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Ku et al., Establishment and characterization of 4 human pancreatic carcinoma cell lines: Genetic alterations in the TGFBR2 gene but not in the MADH4 gene, Cell Tiss Res,2002, 204-214
|
|---|
