SNU-80
| KCLB NO |
00080.1
|
|---|---|
| Name |
SNU-80
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
thyroid
|
|---|---|
| Species |
human, asian female, 59 years old
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
thyroid cancer, anaplastic
|
| Depositor |
professor Jae-Gahb Park
|
| EstablishedBy |
professor Jae-Gahb Park
|
| DateOfUpdated |
01/30/2008
|
| Option |
No Antibiotics
|
| D3S1358 |
15,16
|
|---|---|
| vWA |
16,19
|
| FGA |
20,23
|
| Amelogenin |
X
|
| TH01 |
6,9
|
| TPOX |
8
|
| CSF1P0 |
10,12
|
| D5S818 |
11,13
|
| D13S317 |
8,10
|
| D7S820 |
12,13
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
|
| note |
No antibiotics were added to culture media (No penicillin and streptomycin)
|
| splitratio |
01:03
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
