P3X63Ag8.653
| KCLB NO |
21580.1
|
|---|---|
| Name |
P3X63Ag8.653
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
B cell; B lymphocyte
|
|---|---|
| Species |
mouse
|
| Strain |
BALB/c
|
| Cellular morphology |
lymphoblast
|
| Growth Pattern |
suspension
|
| Histopathology |
myeloma; plasmacytoma
|
| Option |
No Antibiotics
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640, 80%; heat inactivated fetal bovine serum (FBS), 20%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
the cells are resistant to 8-azaguanine and are HAT sensitive; they can be used as fusion partners for producing hybridomas; the cells do not secrete immunoglobulin; the cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity; tested and found negative for ectromelia virus (mousepox)
|
|
J Immunol, 123:1548-1550, 1970; In Vitro Cell Develop Biol, 24:1223-1228, 1988
|
|---|
