NCI-H292
| KCLB NO |
21848.1
|
|---|---|
| Name |
NCI-H292
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human female, 32 years old, Black
|
| VirusSusceptibility |
hepatitis B virus(HBV)
|
| Tumorigenecity |
yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features
|
| Cellular morphology |
epithelial
|
| Production |
keratin; vimentin
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, mucoepidermoid; metastasis to cervical lymph node
|
| Option |
No Antibiotics
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove. Let the culture sit at room temperature or 37
|
|
Banks-Schegel SP et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res, 45:2913-2923, 1985. Yoakum GH et al. High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells. Science 222: 385-389, 1983. Carney DN et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2933, 1985.
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