J11d.2
| KCLB NO |
40183
|
|---|---|
| Name |
J11d.2
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell; B lymphocyte
|
|---|---|
| Species |
rat
|
| Strain |
Lewis(B cell); BALB/c (myeloma)
|
| Isoenzyme |
IgM
|
| Cellular morphology |
lymphoblast
|
| Production |
immunoglobulin; monoclonal antibody; against mouse cortical thymocytes, erythrocytes, neutrophils and B lymphocytes (B cells); cytotoxic
|
| Growth Pattern |
suspension
|
| Histopathology |
hybridoma; mouse(myeloma)
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
MEM, 90%; horse serum, 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| note |
animals were immunized with C3H/HeJ thymocytes; spleen cells were fused with Sp2/0-Ag14 myeloma cells; the antibody does not react with mature (medullary) thymocytes, pluripotential stem cells, platelets or cells of monocyte-macrophage lineage; IgG antibody producing cells are strongly positive for the J11d.2 antigen; tested and found negative for for ectromelia virus (mousepox)
|
|
J Immunol, 127:2496-2501, 1981
|
|---|
