세포주 정보

J1j.10
J1j.10
KCLB NO
40184
Name
J1j.10
세포주 분양조건
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Origin
B cell; B lymphocyte
Species
rat
Strain
Lewis (B cell); BALB/c (myeloma)
Tumorigenecity
yes, in immunosuppressed (B6 X CBA)F1 mice
Isoenzyme
IgM
Cellular morphology
lymphoblast
Production
immunoglobulin; monoclonal antibody; against Thy-1.2 antigen; cytotoxic
Growth Pattern
suspension
Histopathology
hybridoma; mouse(myeloma)
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
MEM, 90%; horse serum, 10%
kclb media
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
note
animals were immunized with C3H/HeJ thymocytes; spleen cells were fused with Sp2/0-Ag14 myeloma cells; the antibody does not lyse Thy-1.1 cells; tested and found negative for ectromelia virus (mousepox)
J immunol, 127; 2496-2501, 1981