HUTU 80
| KCLB NO |
30040
|
|---|---|
| Name |
HUTU 80
|
세포주 분양조건
서비스
수량
-
+
| Origin |
duodenum
|
|---|---|
| Species |
human male, 53 years old, Caucasian
|
| Receptor |
Receptor for bombesin at up to 6000 sites per cell
|
| Tumorigenecity |
Yes, in nude mice; forms well differentiated papillary adenocarcinoma, (grade I).
|
| Isoenzyme |
PGM3,1-2;PGM1,1-2;ES-D,1;Me-2,2;AK-1,1;GLO-1,2;G6PD,B
|
| Karyology |
(P12) hypodiploid to hyperdiploid
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma
|
| Modal Choromosome No |
46
|
| D3S1358 |
15,17
|
|---|---|
| vWA |
16,18
|
| FGA |
21,23
|
| Amelogenin |
X,Y
|
| TH01 |
7
|
| TPOX |
9,11
|
| CSF1P0 |
11,13
|
| D5S818 |
12,13
|
| D13S317 |
8,11
|
| D7S820 |
9,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium (Eagle) in Earle
|
| kclb media |
Dulbecco
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
| splitratio |
1:2 to 1:5 is recommended.
|
| media change |
2 to 3 times weekly
|
