145-2C11
| KCLB NO |
21975
|
|---|---|
| Name |
145-2C11
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell; B lymphocyte
|
|---|---|
| Species |
hamster
|
| Strain |
?(B cell); BALB/c(myeloma)
|
| Cellular morphology |
lymphoblast
|
| Production |
Immunoglobulin; monoclonal antibody; against mouse CD3
|
| Growth Pattern |
suspension
|
| Histopathology |
hybridoma; mouse(myeloma)
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Iscove's modified Dulbecco's medium, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2x10 exp5 cells/ml and maintain between 1x10 exp5 and 1x10 exp6 cells/ml.
|
| media change |
every 2 to 3 days
|
|
Leo O et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc.Natl.Acad.Sci.USA 84:1374-1378, 1987Kayagaki N et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc.Natl.Acad.Sci.USA 94:3914-3919, 1997
|
|---|
