NCI-H498
| KCLB NO |
10254
|
|---|---|
| Name |
NCI-H498
|
세포주 분양조건
서비스
수량
-
+
| Origin |
colon; colorectal; cecal
|
|---|---|
| Species |
human male, 56years old, Caucasian
|
| Reverse Transcriptase |
negative
|
| Tumorigenecity |
yes, in nude mice
|
| Isoenzyme |
G6PD, B;PGM1, 1;PGM3, 1;ES-D, 1;AK-1 , 1;Me-2, 1;GLO-1, 2
|
| Cellular morphology |
epithelial
|
| Production |
Carcinoembryonic antigen, 826 ng/ml per 10 exp6 cells per 10 days; mucin
|
| Growth Pattern |
floating aggregates of round cells with some attached cells
|
| Histopathology |
adenocarcinoma
|
| Modal Choromosome No |
51 (47 to 54)
|
| Depositor |
Adi F Gazdar
|
| D3S1358 |
15
|
|---|---|
| vWA |
15
|
| FGA |
22,24
|
| Amelogenin |
X
|
| TH01 |
7
|
| TPOX |
8
|
| CSF1P0 |
12
|
| D5S818 |
13
|
| D13S317 |
9,11
|
| D7S820 |
8,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Cultures can be maintained by additional or replacement of fresh medium.Alternatively, the cells may be collected by centrifugation and then resuspended at 20000 to 40000 viable cells/ml.When the cell concentration becomes heavy with large clumps, aspirate well and dilute approximately 1:10If the clumps are allowed to get too large, most of the cells will die
|
| note |
The line was derived from metastasis in the peritoneum obtained from a patient after treatment prior to therapy.The cells are glandular structures and secrete mucin.The cells are positive for Dopa decarboxylase , CA19-9 and TAG-72 antigens and CEA.Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetyl-galacto-aminyl-transferase activity
|
|
Park JG et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47:6710-6718, 1987. Dahiya R et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49:4550-4556, 1989.
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