세포주 정보

RR1022
RR1022
KCLB NO
10047
Name
RR1022
세포주 분양조건
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Origin
Species
rat
Strain
Amsterdam
VirusSusceptibility
vesicular stomatitis(Indiana)
Virus Resistance
poliovirus1, 2, 3
Reverse Transcriptase
positive
Tumorigenecity
yes, in newborn rats and chickens
Cellular morphology
epithelial
Growth Pattern
monolayer
Histopathology
sarcoma
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
Medium 199 in Hank's BBS , 80%; heat inactivated bovine calf serum (BCS), 20%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove medium, add fresh 0.25 % trypsin for 1 to 3 minutes, remove medium allow the culture to sit at room temp. For 5 to 10 min. Add fresh medium, aspirate and dispense into new flasks
Hereditas 50: 53-80, 1963