NCI-H548
| KCLB NO |
10249
|
|---|---|
| Name |
NCI-H548
|
세포주 분양조건
서비스
수량
-
+
| Origin |
colon
|
|---|---|
| Species |
human male, 52 years old, Caucasian
|
| Reverse Transcriptase |
negative
|
| Tumorigenecity |
yes, in nude mice
|
| Isoenzyme |
G6PD, B; PGM1, 1 ;PGM3, 1;ES-D, 1;AK-1, 1-2;GLO-1, 2
|
| Karyology |
the Y chromosome is not present in most of the cells
|
| Cellular morphology |
epithelial
|
| Production |
carcinoembryonic antigen, 18ng/ml per 10 exp6 cells per 10 days
|
| Growth Pattern |
floating aggregates of round cells with some attached cells
|
| Histopathology |
adenocarcinoma
|
| Modal Choromosome No |
59 (55 to 63)
|
| D3S1358 |
16
|
|---|---|
| vWA |
15,18
|
| FGA |
21,22
|
| Amelogenin |
X
|
| TH01 |
7
|
| TPOX |
10,11
|
| CSF1P0 |
11
|
| D5S818 |
12,13
|
| D13S317 |
9,11
|
| D7S820 |
9,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
ACL-4 medium with modifications
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Cultures can be maintained by addition or replacement of fresh medium.Alternatively, scrape off the attached cells and transfer along with the floating cells into new flasks.Add medium as acidity increases, and divide as the density of the culture increase
|
| note |
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The line was obtained from a well differentiated sigmoid tumor from a patient prior to therapy. The line was initially grown in medium with fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4. Floating aggregates produce tubuloglandular structures lined by columnar epithelia. When grown in medium with serum, the cell attach partially to the substrate and form domes. The cells and tumors formed from the cells are positive for Dopa decarboxylase and are negative for the TAG-72 antigen.
|
|
Park JG et al. Characteristics of cell lines established from human colorectal carcinoma, Cancer Res. 47: 6710-6718, 1987.
|
|---|
