M2-10B4
| KCLB NO |
21972
|
|---|---|
| Name |
M2-10B4
|
세포주 분양조건
서비스
수량
-
+
| Origin |
metastasis to bone marrow; stromal
|
|---|---|
| Species |
mouse
|
| Cellular morphology |
fibroblast
|
| Production |
laminin; collagen 4
|
| Growth Pattern |
monolayer
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove spent medium, add 0.25% trypsin solution, rinse and remove trypsin. Keep the cells at room temperature until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
|
| note |
The M2-10B4 cell line is a clone derived from bone marrow stromal cells from a (C57BL/6J X C3H/HeJ) F1 mouse.
The cells express laminin and collagen 4 , but do not express collagen 1 or factor 8
The cells will support human and murine myelopoiesis in long term culture, and will support certain murine stromal cell dependent pre-B cell lines in vitro.
|
|
Sutherland HJ et al .Difeerential regulation of primitive human hematopoietic cells in long-term cultures maintained on genetically engineered murine stromal cells. Blood 78:666-672, 1991.
|
|---|
