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SNU-5

KCLB NO	00005
Name	SNU-5

세포주 분양조건

비영리 동결(Frozen)
비영리 배양(Culture)
영리 동결(Frozen)
영리 배양(Culture)

서비스

DNA extraction service
cDNA synthesis service
Pellet service

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Origin	stomach; ascites
Species	human, asian female, 33 years old
Abnormal genes	p53: codon 262-269, 24bp deletion, 8 amino acids
Cellular morphology	lymphoblast-like
Growth Pattern	suspension
Histopathology	adenocarcinoma
Differentiation	poorly differentiated
Depositor	Jae-Gahb Park, M.D.
DateOfUpdated	2014-09-11

D3S1358	16
vWA	15,16
FGA	21
Amelogenin	X
TH01	9
TPOX	11
CSF1P0	12
D5S818	10
D13S317	8
D7S820	8,12

준비중입니다.

freeze media	RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing	Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
note	Penicillin-streptomycin were added to culture media (final concentration, 100 U/mL)
populaton doubling time	34 hours
splitratio	1:4
media change	Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days

Cancer Res, 50: 2773-2780, 1990; J Nat
Shin KH, Yang YM, Park JG: Absence or decreased levels of the hMLH1 protein in human gastric carcinoma cell lines: implication of hMLH1 in alkylation tolerance. J Cancer Res Clin Oncol 124 8 421-6 ▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 00216, 00484, 00520, 00601, 00620, 00638, 00668, 00719 ] Kim DK, Kim HT, Tai JH, Cho YB, Kim TS, Kim KH, Park JG, Hong WS: Pharmacokinetics and antitumor activity of a new platinum compound, cis-malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1, 3- dioxolane]platinum(II), as determined by ex vivo pharmacodynamics. Cancer Chemother Pharmacol 37 1-2 1-6 1995 ▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 80103, 30103, {PC-9}, {PC-14} ] Park IK, Kim BJ, Goh YJ, Lyu MA, Park CG, Hwang ES, Kook YH: Co-expression of urokinase-type plasminogen activator and its receptor in human gastric-cancer cell lines correlates with their invasiveness and tumorigenicity. Int J Cancer 29 71(5) 867-73 May 1997 ▶ ref. kclb cell line no.'s - [ 00001, 00005, 21736, {Hs746T} ] Bae CD, Park SE, Seong YS, Kimm SW, Park JB, Park JB: The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines. J Korean Med Sci 8 2 153-9 Apr 1993 ▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 30103 ] Choe G, Kim WH, Park JG, Kim YI: Effect of suramin on differentiation of human stomach cancer cell lines. J Korean Med Sci 12 5 433-42 Oct 1997 ▶ ref. kclb cell line no.'s - [ 00005, 00016 ] Jung HC, Kim JM, Song IS, Kim CY: Helicobacter pylori induces an array of pro-inflammatory cytokines in human gastric epithelial cells: quantification of mRNA for interleukin-8, -1 alpha/beta, granulocyte-macrophage colony-stimulating factor, monocyte chemoattractant protein-1 and tumour necrosis factor-alpha. J Gastroenterol Hepatol 12 7 473-80 Jul 1996 ▶ ref. kclb cell line no.'s - [ 00005, 30103 ] Buell JF, Reed E, Lee KB, Parker RJ, Venzon DJ, Amikura K, Arnold S, Fraker DL, Alexander HR: Synergistic effect and possible mechanisms of tumor necrosis factor and cisplatin cytotoxicity under moderate hyperthermia against gastric cancer cells. Ann Surg Oncol 4 2 141-8 Mar 1997 ▶ ref. kclb cell line no.'s - [ 00005 ]

Cancer Res, 50: 2773-2780, 1990; J Nat

Shin KH, Yang YM, Park JG: Absence or decreased levels of the hMLH1 protein in human gastric carcinoma cell lines: implication of hMLH1 in alkylation tolerance. J Cancer Res Clin Oncol 124 8 421-6
▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 00216, 00484, 00520, 00601, 00620, 00638, 00668, 00719 ]
Kim DK, Kim HT, Tai JH, Cho YB, Kim TS, Kim KH, Park JG, Hong WS: Pharmacokinetics and antitumor activity of a new platinum compound, cis-malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1, 3- dioxolane]platinum(II), as determined by ex vivo pharmacodynamics. Cancer Chemother Pharmacol 37 1-2 1-6 1995
▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 80103, 30103, {PC-9}, {PC-14} ]
Park IK, Kim BJ, Goh YJ, Lyu MA, Park CG, Hwang ES, Kook YH: Co-expression of urokinase-type plasminogen activator and its receptor in human gastric-cancer cell lines correlates with their invasiveness and tumorigenicity. Int J Cancer 29 71(5) 867-73 May 1997
▶ ref. kclb cell line no.'s - [ 00001, 00005, 21736, {Hs746T} ]
Bae CD, Park SE, Seong YS, Kimm SW, Park JB, Park JB: The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines. J Korean Med Sci 8 2 153-9 Apr 1993
▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 30103 ]
Choe G, Kim WH, Park JG, Kim YI: Effect of suramin on differentiation of human stomach cancer cell lines. J Korean Med Sci 12 5 433-42 Oct 1997
▶ ref. kclb cell line no.'s - [ 00005, 00016 ]
Jung HC, Kim JM, Song IS, Kim CY: Helicobacter pylori induces an array of pro-inflammatory cytokines in human gastric epithelial cells: quantification of mRNA for interleukin-8, -1 alpha/beta, granulocyte-macrophage colony-stimulating factor, monocyte chemoattractant protein-1 and tumour necrosis factor-alpha. J Gastroenterol Hepatol 12 7 473-80 Jul 1996
▶ ref. kclb cell line no.'s - [ 00005, 30103 ]
Buell JF, Reed E, Lee KB, Parker RJ, Venzon DJ, Amikura K, Arnold S, Fraker DL, Alexander HR: Synergistic effect and possible mechanisms of tumor necrosis factor and cisplatin cytotoxicity under moderate hyperthermia against gastric cancer cells. Ann Surg Oncol 4 2 141-8 Mar 1997
▶ ref. kclb cell line no.'s - [ 00005 ]

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