Hs 746T
| KCLB NO |
30135.1G
|
|---|---|
| Name |
Hs 746T
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
stomach
|
|---|---|
| Species |
|
| Tumorigenecity |
yes, in immunosuppressed mice; forms carcinomas
|
| Isoenzyme |
G6PD, B; PGM1, 1-2; PGM3, 1; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1
|
| Karyology |
(P26) hyperdiploid with mode=70, Y chromosomes present
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, metastatic to left leg
|
| Option |
No Antibiotics
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco
|
| kclb media |
DMEM, 90%; fetal bovine serum (FBS), 10%
|
| subculturing |
the Hs 746T line was isolated at the Naval Biosciences Laboratory in 1973 from a stomach carcinoma metastatic to the leg of a 74 year old male caucasian. In vitro transformation properties were assayed at various early passages. Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts
|
