세포주 정보

Hs 294T
Hs 294T
KCLB NO
30140.1G
Name
Hs 294T
세포주 분양조건
  • 무항생제 배양
서비스
수량
- +
Origin
skin
Species
human male, 56 years old, caucasian
Receptor
nerve growth factor; interferon
Tumorigenecity
yes, in immunosuppressed NAMRU mice
Isoenzyme
G6PD, B; PGM1, 1-2; PGM3, 1; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 1-2
Karyology
(P26) heteroploid with modal number=59; Y chromosomes present
Cellular morphology
mixed stellate and polygonal
Growth Pattern
monolayer
Histopathology
melanoma; metastatic to lymph node
Option
No Antibiotics
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
kclb media
DMEM 90%; heat inactivated fetal bovine serum (FBS), 10%
note
the proliferative of the cell ine is enhanced by addition of retinoic acid; responds to mitogenic stimulation by phytohemagglutinin and forms colonies in soft agar medium or on contact inhibited monolayers. The melanoma cells have also been shown to be responsive to the anti proliferative activity of interferon; results from a competitive binding study indicated that Hs 294T cells possess at least two types of interferon (IFN) receptors, one which binds IFN alpha and IFN beta and another which binds IFN gamma
In Vitro, 15:342-350, 1979; In Vitro, 15;565-568, 1979