SNU-475
| KCLB NO |
00475
|
|---|---|
| Name |
SNU-475
|
세포주 분양조건
서비스
수량
-
+
| Origin |
liver
|
|---|---|
| Species |
human, asian male, 43 years old
|
| Virus |
Hepatitis B virus (HBV)
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, hepatocellular; primary
|
| Depositor |
Jae-Gahb Park, M.D.
|
| D3S1358 |
14
|
|---|---|
| vWA |
14
|
| FGA |
23
|
| Amelogenin |
X,Y
|
| TH01 |
7,9
|
| TPOX |
8,9
|
| CSF1P0 |
11,12
|
| D5S818 |
10,13
|
| D13S317 |
8,11
|
| D7S820 |
7,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
| note |
Hepatitis B virus integrated.
|
| populaton doubling time |
66 hours
|
| splitratio |
1:4
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Park et al., Characterization of cell lines established from human hepatocellular carcinoma. Int J Cancer, 62: 276-282, 1995; Kang et al., Mutation of p53 gene in hepatocellular carcinoma cell lines with HBX DNA. Int. J. Cancer, 67: 898-902, 1996
|
|---|
