WEHI164
| KCLB NO |
21751
|
|---|---|
| Name |
WEHI164
|
세포주 분양조건
서비스
수량
-
+
| Origin | |
|---|---|
| Species |
mouse
|
| Strain |
BALB/c
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
fibrosarcoma
|
| Option |
culture or frozen
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
this line was originally established by M. Rollinghoff and N.L. Warner from a fibrosarcoma induced by subcutaneous injections of 3-methylcholanthrene; after pretreatment with actinomycin D, the cells are highly sensitive to human cytotoxic monocytes, to human tumor necrosis factor and to lymphotoxin; they can be used as target cells for short term chromium release cytotoxicity assays; tested and found negative for ectromelia virus (mousepox)
|
|
Proc Soc Exp Biol Med, 144:813-818, 1973; J Nat'l Cancer Inst, 72:23-29, 1984; Blood, 65:8-14, 1985
|
|---|
