SK-MEL-3
| KCLB NO |
30069
|
|---|---|
| Name |
SK-MEL-3
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lymph node
|
|---|---|
| Species |
human female, 42 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice; forms pigmented malignant melanoma
|
| Isoenzyme |
PGM3,1;PGM1,1-2;ES-D,1;AK-1,1;GLO-1,2;G6PD,B
|
| Karyology |
(P13) hypotetraploid to hypertetraploid with abnormalities
|
| Cellular morphology |
fibroblast
|
| Growth Pattern |
monolayer
|
| Histopathology |
malignant melanoma; skin; from metastatic site
|
| D3S1358 |
17,18
|
|---|---|
| vWA |
14,18
|
| FGA |
24
|
| Amelogenin |
X
|
| TH01 |
6
|
| TPOX |
8
|
| CSF1P0 |
9,10
|
| D5S818 |
11
|
| D13S317 |
12,13
|
| D7S820 |
8,10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
McCoy
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach (about 10min).Add fresh medium, aspirate and dispense into new flasks.Subculture every 6 to 8 days.
|
| splitratio |
1:3 to 1:5
|
| media change |
2 to 3 times per week
|
|
Fogh J., ed., Human tumor cells in vitro. New York: Plenum Press; 1975:pp. 115-159. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One humdred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
