세포주 정보

HCT 116
HCT 116
KCLB NO
10247
Name
HCT 116
세포주 분양조건
서비스
수량
- +
Origin
colon; colorectal
Species
human male
Reverse Transcriptase
negative
Tumorigenecity
yes, in nude mice
Isoenzyme
G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1-2; AK-1, 1; GLO-1, 1
Cellular morphology
epithelial
Production
carcinoembryonic antigen (CEA) 1 ng per 10exp6 cells per 10 days; keratin
Growth Pattern
monolayer
Histopathology
carcinoma
D3S1358
12,19
vWA
17,22
FGA
18,23
Amelogenin
X,Y
TH01
8,9
TPOX
8
CSF1P0
7,10
D5S818
10,11
D13S317
10,12
D7S820
11,12
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
McCoy
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
note
the cells are positive for keratin by immunoperoxidase staining; growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts
Cancer Res, 41:1751-1756, 1981
  • Shin KH, Han HJ, Park JG: Growth suppression mediated by transfection of wild-type hMLH1 in human cancer cells expressing endogenous truncated hMLH1 protein. Int J Oncol 12 3 609-15 Mar 1998
    ▶ ref. kclb cell line no.'s - [ 00001, 10247 ]
  • Yee LK, Allegra CJ, Trepel JB, Grem JL: Metabolism and RNA incorporation of cyclopentenyl cytosine in human colorectal cancer cells. Biochem Pharmacol 1 43(7) 1587-99 Apr 1992
    ▶ ref. kclb cell line no.'s - [ 0000C4, 10247, 90630 ]
  • Grem JL, Allegra CJ: Sequence-dependent interaction of 5-fluorouracil and arabinosyl-5-azacytosine or 1-beta-D-arabinofuranosylcytosine. Biochem Pharmacol 5 42(2) 409-18 Jul 1991
    ▶ ref. kclb cell line no.'s - [ 0000C4, 10247 ]