L6
| KCLB NO |
21458
|
|---|---|
| Name |
L6
|
세포주 분양조건
서비스
- 서비스 준비중입니다.
수량
-
+
| Origin |
muscle; skeletal muscle
|
|---|---|
| Species |
rat
|
| Cellular morphology |
myoblast
|
| Production |
myosin
|
| Growth Pattern |
monolayer
|
| Histopathology |
normal
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco's modified Eagle's Medium, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| note |
L6 cells fuse in culture to form multinucleated myotubes and striated fibers. The extent of cell fusion declines with passage and the cells should be frozen at low passage and periodically recloned with selection for fusion competent cells; the myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent; tested and found negative for ectromelia virus (mousepox)
|
|
Proc Nat'l Acad Sci, USA, 61:477-483, 1968; Dev Biol, 23:1-22, 1970; Nature, 251:618-620, 1974
|
|---|
