세포주 정보

L5178-S(LY-S)
L5178-S(LY-S)
KCLB NO
21723
Name
L5178-S(LY-S)
세포주 분양조건
서비스
수량
- +
Origin
thymus
Species
mouse
Strain
DBA/2
Tumorigenecity
yes, in DBA/2 mice (but only weakly)
Cellular morphology
lymphoblast
Growth Pattern
suspension
Histopathology
lymphoma; chemically induced
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
Fischer's medium with 0.1g/L sodium pyruvate and 1.125g/L sodium bicarbonate, 90%; horse serum, 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
note
this line was derived from the X-ray resistant L5178-R line (KCLB 21722); the cells are sensitive to X-rays, ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD); the parental cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse; tested and found negative for ectromelia virus (mousepox)
Cancer Res, 43:4736-4742, 1983; Brit J Cancer, 49:107-111; Int J Radiat, 48:609-619, 1985