Calu-3
| KCLB NO |
30055
|
|---|---|
| Name |
Calu-3
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human male, 25 years old, Caucasian
|
| Tumorigenecity |
Yes, forms well differentiated grade I adenocarcinoma in nude mice
|
| Isoenzyme |
Me-2,1; PGM3, 1-2; PGM1,1; ES D, 1; AK1,1;GLO-1,1-2;G6PD,B
|
| Karyology |
The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma; pleural effusion
|
| D3S1358 |
15,18
|
|---|---|
| vWA |
16,17
|
| FGA |
25
|
| Amelogenin |
X
|
| TH01 |
6,9.3
|
| TPOX |
8
|
| CSF1P0 |
11,12
|
| D5S818 |
11
|
| D13S317 |
12
|
| D7S820 |
10,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium (Eagle) in Earle
|
| kclb media |
Dulbecco
|
| subculturing |
Remove medium, add with fresh 0.25% trypsin solution for 1 min, remove trypsin and let the culture sit at room temperature for 5 to 10 mins. Add fresh medium, aspirate and dispense into new flasks.Subculture every 6 to 8 days
|
| splitratio |
1:3 to 1:6
|
| media change |
2 to 3 times per week
|
|
J.Fogh and G.Trempe, In: Human Tumor Cells In Vitro, PP.115-159, J. Fogh(ed), Plenum Press, New York, 1975. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One humdred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
