세포주 정보

SNU-3173
SNU-3173
KCLB NO
03173
Name
SNU-3173
세포주 분양조건
서비스
수량
- +
Origin
lung
Species
human, asian male, 46 years old
Cellular morphology
epithelial
Growth Pattern
monolayer
Option
culture only
D3S1358
17
vWA
16
FGA
23,24
Amelogenin
X,Y
TH01
9
TPOX
9,11
CSF1P0
11,12
D5S818
10,12
D13S317
10
D7S820
8
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
subculturing
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
note
EGFR exon 20 insertion mutation / EGFR H773insAH
Preclinical Modeling of Osimertinib for NSCLC With EGFR Exon 20 Insertion Mutations., J Thorac Oncol. 2019 Sep;14(9):1556-1566.