GK1.5
| KCLB NO |
40207
|
|---|---|
| Name |
GK1.5
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell; B lymphocyte
|
|---|---|
| Species |
rat
|
| Strain |
Lewis (B cell); BALB/c (myeloma)
|
| Isoenzyme |
IgG2b
|
| Cellular morphology |
lymphoblast
|
| Production |
immunoglobulin; monoclonal antibody; against mouse helper, inducer T cells (L3T4 antigen, CD4)
|
| Growth Pattern |
suspension
|
| Histopathology |
hybridoma; mouse(myeloma)
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco's modified Eagle's Medium, with 4.5g/L glucose, 80%; heat inactivated fetal bovine serum (FBS), 20% or Iscove's modified Dulbecco's medium, 80%; heat inactivated fetal bovine serum (FBS), 20%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
animals were immunized with the cloned cytotoxic T lymphocyte lines V4 and 243/2.5; spleen cells were fused with Sp2/0-Ag14 myeloma cells; L3T4 is expressed on mouse helper/induce T cells and is analogous to the human Leu-3/T4 molecule; the antibody profoundly blocks antigen specific murine class II MHC antigen reactive helper I lymphocyte lines; tested and found negative for ectromelia virus (mousepox)
|
|
Immunol Rev, 74:29-56, 1983; J Immunol, 131:2178-2183, 1983; J Immunol, 131:2445-2451, 1983
|
|---|
