SK-N-SH
| KCLB NO |
30011
|
|---|---|
| Name |
SK-N-SH
|
세포주 분양조건
서비스
수량
-
+
| Origin |
brain
|
|---|---|
| Species |
human female, 4 years old
|
| Isoenzyme |
Me-2,2;PGM3,1;PGM1,1;ES D,1;AK1,1;GLO-1,1;G6PD,B.
|
| Karyology |
hypodiploid to hyperdiploid; no abnormalities observed
|
| Cellular morphology |
epithelial
|
| Production |
plasminogen activator
|
| Growth Pattern |
monolayer
|
| Histopathology |
Neuroblastoma, metastasis to bone marrow
|
| Modal Choromosome No |
47
|
| D3S1358 |
15,16
|
|---|---|
| vWA |
14,18
|
| FGA |
23.2,24
|
| Amelogenin |
X
|
| TH01 |
7,10
|
| TPOX |
8,11
|
| CSF1P0 |
11
|
| D5S818 |
12
|
| D13S317 |
11
|
| D7S820 |
7,10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Eagle
|
| kclb media |
MEM with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
MEM with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
The SK-N-SH line differs from SK-N-MC in that it exhibits a longer doubling time and high levels of dopamine-beta-hydroxylase.
|
|
Gilbert LC and Wachsman JT. Characterization and partial purification of the plasminogen activator from human neuroblastoma cell line, SK-N-SH. A comparison with human urokinase. Biochim. Biophys.Acta 704: 450-460, 1982Spengler BA. et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells estaablished in vitro.In Vitro 8: 410, 1973Bluestain HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978
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