세포주 정보

SK-BR-3
SK-BR-3
KCLB NO
30030
Name
SK-BR-3
세포주 분양조건
서비스
수량
- +
Origin
breast, mammary gland
Species
human female, 43 years old, Caucasian
Tumorigenecity
Yes, in nude mice; forms poorly differentiated adenocarcinoma
Isoenzyme
PGM3,1;PGM1,1-2;ES-D,1;AK-1,1-2;GLO-1,2;G6PD,B
Cellular morphology
epithelial
Growth Pattern
monolayer
Histopathology
adenocarcinoma; pleural effusion, malignant
D3S1358
17
vWA
17
FGA
20
Amelogenin
X
TH01
8,9
TPOX
8,11
CSF1P0
12
D5S818
9,12
D13S317
11,12
D7S820
9,12
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
McCoy
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove medium, rinse with fresh 0.25 % trypsin solution , remove trypsin and let the culture sit at room temperature (or at 37
Fogh, J., ed., Human tumor cells in vitro. New York: Plenm Press; 1975:pp.115-159Trempe GL. Human breast cancer in culture. Recent Results Cancer Res. :33-341, 1976