Calu-6
| KCLB NO |
30056
|
|---|---|
| Name |
Calu-6
|
세포주 분양조건
서비스
수량
-
+
| Origin |
unknown; probably lung
|
|---|---|
| Species |
human female, 61 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice; forms poorly differentiated carcinoma
|
| Isoenzyme |
Me-2,1;PGM3,1;PGM1,2;ES-D,1;AK-1,1;GLO-1,2;G6PD,B
|
| Karyology |
The stemline chromosome number is hypotriploid and the 2S component occurred at 5.8%.
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, anaplastic
|
| Differentiation |
anaplastic
|
| D3S1358 |
16
|
|---|---|
| vWA |
17
|
| FGA |
22
|
| Amelogenin |
X
|
| TH01 |
9
|
| TPOX |
8
|
| CSF1P0 |
12
|
| D5S818 |
11
|
| D13S317 |
11
|
| D7S820 |
10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium (Eagle) in Earle
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach (about 10min).Add fresh medium, aspirate and dispense into new flasks.Subculture every 6 to 8 days.
|
| splitratio |
1:2 to 1:8
|
| media change |
2 to 3 times per week
|
|
J.Fogh and G.Trempe, In: Human Tumor Cells In Vitro, PP.115-159, J. Fogh(ed), Plenum Press, New York, 1975. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One humdred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
