U-373 MG
| KCLB NO |
30017
|
|---|---|
| Name |
U-373 MG
|
세포주 분양조건
서비스
수량
-
+
| Origin |
brain
|
|---|---|
| Species |
human male, 61 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice; Grade III astrocytomas are formed
|
| Isoenzyme |
PGM3,1;PGM1,1;ES-D,1;G6PD,B;AK-1,1;GLO-1,1
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
glioblastoma; astrocytoma
|
| D3S1358 |
16,17
|
|---|---|
| vWA |
16,18
|
| FGA |
21,25
|
| Amelogenin |
X,Y
|
| TH01 |
9.3
|
| TPOX |
8
|
| CSF1P0 |
11,12
|
| D5S818 |
11,12
|
| D13S317 |
10,11
|
| D7S820 |
10,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium Eagle with 2mM L-glutamine and Earle
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin solution for 1 minute , remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
|
Beckman G et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by Hela cells. Hum.Hered. 21:238-241, 1971Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58:209-214, 1977
|
|---|
