A-704
| KCLB NO |
30045
|
|---|---|
| Name |
A-704
|
세포주 분양조건
서비스
수량
-
+
| Origin |
kidney
|
|---|---|
| Species |
human male, 78 years old
|
| Tumorigenecity |
No
|
| Isoenzyme |
Me-2,1;PGM3.1-2;PGM1,1;ES-D,1;AK-1,1;GLO-1,2;G6PD,B
|
| Karyology |
(P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dendritics and endoreduplication
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma
|
| D3S1358 |
15
|
|---|---|
| vWA |
14,18
|
| FGA |
22,23
|
| Amelogenin |
X,Y
|
| TH01 |
7,9
|
| TPOX |
11
|
| CSF1P0 |
7,8
|
| D5S818 |
10,11
|
| D13S317 |
8
|
| D7S820 |
10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium Eagle in Earle
|
| kclb media |
Dulbecco
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
| splitratio |
1:3 to 1:4
|
| media change |
2 to 3 times weekly
|
|
Giard DJ at al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J.Natl.Cancer Inst. 51: 1417-1423, 1973
|
|---|
