NCI-H69
| KCLB NO |
30119
|
|---|---|
| Name |
NCI-H69
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human male, 55 years old, Caucasian
|
| Tumorigenecity |
yes, in nude mice; forms tumors with typical small cell carcinoma histology
|
| Isoenzyme |
G6PD, B; PGM1, 2; PGM3, 1;ES-D, 2; Me-2, 1; AK-1, 1; GLO-1, 1-2
|
| Karyology |
aneuploid, with 3p deletion; range=40 to 73
|
| Cellular morphology |
floating aggregates
|
| Growth Pattern |
suspension
|
| Histopathology |
small cell lung cancer (SCLC)
|
| D3S1358 |
16
|
|---|---|
| vWA |
16,17
|
| FGA |
24
|
| Amelogenin |
X,Y
|
| TH01 |
8,9
|
| TPOX |
10
|
| CSF1P0 |
10,12
|
| D5S818 |
11,13
|
| D13S317 |
12
|
| D7S820 |
9
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640, 90%; heat inactivated fetal bovine serum (FBS), 10% or Iscove
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
this cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics; the cells grow in aggregates, thus cell counts are not accurate; the line can be adapted to grow in shaker flask or spinner flask systems
|
|
Cance Res, 40;3502-3507, 1980
|
|---|
