Caki-1
| KCLB NO |
30046
|
|---|---|
| Name |
Caki-1
|
세포주 분양조건
서비스
수량
-
+
| Origin |
kidney
|
|---|---|
| Species |
human male, 49 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters
|
| Isoenzyme |
Me-2,2;PGM3,1-2;PGM1,1;ES-D,1-2;AK-1,1;GLO-1,1-2;G6PD,B
|
| Karyology |
(P4)hypertriploid (+A2,+B,+C,+D,+E,+F,+G) with abnormalities
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, clear cell; metastasis to skin
|
| D3S1358 |
17
|
|---|---|
| vWA |
15,17
|
| FGA |
26
|
| Amelogenin |
X
|
| TH01 |
6,8
|
| TPOX |
8,11
|
| CSF1P0 |
10,11
|
| D5S818 |
11,12
|
| D13S317 |
11,12
|
| D7S820 |
8,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
McCoy
|
| kclb media |
Dulbecco
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
| splitratio |
1:2 to 1:4
|
| media change |
2 to 3 times weekly
|
|
Fogh J et al. Absence of Hela cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cacer Inst. 59: 221-226, 1977
|
|---|
