ME-180
| KCLB NO |
30033
|
|---|---|
| Name |
ME-180
|
세포주 분양조건
서비스
수량
-
+
| Origin |
cervix, uterine
|
|---|---|
| Species |
human female, 66 years old, Caucasian
|
| Virus |
The cells contain human papillomavirus(HPV) DNA with greater homology to HPV-39 than HPV-18
|
| Tumorigenecity |
Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I)
|
| Isoenzyme |
PGM3,1;PGM1,1-2;ES-D,1-2;AK-1,1;GLO-1,2;G6PD,B
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma; epidermoid; from metastatic site: omentum
|
| D3S1358 |
16
|
|---|---|
| vWA |
15,17
|
| FGA |
23
|
| Amelogenin |
X
|
| TH01 |
8,9.3
|
| TPOX |
8,10
|
| CSF1P0 |
11
|
| D5S818 |
12
|
| D13S317 |
11,13
|
| D7S820 |
9,10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
McCoy's 5a medium, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse with fresh 0.25 % trypsin solution , remove trypsin and let the culture sit at room temperature (or at 37''''C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
|
Sugarman BJ et al. Recombiant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
