NIH3T3
| KCLB NO |
21658
|
|---|---|
| Name |
NIH3T3
|
세포주 분양조건
서비스
수량
-
+
| Origin |
embryo
|
|---|---|
| Species |
mouse
|
| Strain |
NIH/Swiss
|
| Virus |
murine sarcoma viruses; murine leukemia viruses
|
| Cellular morphology |
fibroblast
|
| Growth Pattern |
monolayer
|
| Option |
culture or frozen
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco
|
| kclb media |
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated bovine calf serum (BCS), 10%
|
| note |
these cells are useful for DNA transfection and transformation studies; tested and found negative for ectromelia virus (mousepox). Fetal calf serum is no suitable. The serum initially employed and found to be satisfactory was from the Colorado Serum Company
|
|
J Virol, 4:549-553, 1969
|
|---|
