MCF-7
| KCLB NO |
30022
|
|---|---|
| Name |
MCF-7
|
세포주 분양조건
서비스
수량
-
+
| Origin |
breast, mammary gland
|
|---|---|
| Species |
human female, 69 years old, Caucasian
|
| Receptor |
estrogen
|
| Isoenzyme |
PGM3,1;PGM1,1-2;ES-D,1-2;AK-1,1;GLO-1,1-2;G6PD,
|
| Cellular morphology |
epithelial
|
| Production |
Insulin-like growth factor binding proteins(IGFBP) BP-2;BP-4;BP-5
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma; pleural effusion
|
| Requirement |
supplemented with 0.01mg/ml bovine insulin
|
| D3S1358 |
16
|
|---|---|
| vWA |
14,15
|
| FGA |
23,25
|
| Amelogenin |
X
|
| TH01 |
6
|
| TPOX |
9,12
|
| CSF1P0 |
10
|
| D5S818 |
11,12
|
| D13S317 |
11
|
| D7S820 |
8,9
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium Eagle with 2mM L-glutamine and Earle
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin- 0.03 % EDTA solution for 1 minute , remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
|
Sugarman BJ et al. Recombiant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985Pratt SE and Pollak MN. Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-bindning proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993
|
|---|
