세포주 정보

HL-60
HL-60
KCLB NO
10240.1G
Name
HL-60
세포주 분양조건
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수량
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Origin
blood
Species
human human
Reverse Transcriptase
negative
Isoenzyme
G6PD,B;PGM1,1;PGM3,1;ES-D,1;Me-2,1;AK-1,1;GLO-1,1
Cellular morphology
lymphoblast
Production
tumor necrosis factor(TNF), also known as tumor necrosis factor alpha(TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid
Growth Pattern
suspension
Histopathology
leukemia, acute promyelocytic
Requirement
complement; Fc
Option
No Antibiotics
D3S1358
16
vWA
16
FGA
22,24
Amelogenin
X
TH01
7,8
TPOX
8,11
CSF1P0
13,14
D5S818
12
D13S317
8,11
D7S820
11,12
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
note
HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid(PMA, TPA), dimethylsulfoxide(DMSO), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli; the line contains the myc oncogene
Nature 270:347-349, 1977; Nature 312:724-729, 1984; J.Biol.Chem. 260:2345-2354, 1985