SK-MEL-1
| KCLB NO |
30067
|
|---|---|
| Name |
SK-MEL-1
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lymphatic system
|
|---|---|
| Species |
human male, 28 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice; forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters
|
| Isoenzyme |
PGM3,1;PGM1,1;ES-D,1;AK-1,1;GLO-1,1-2;G6PD,B
|
| Cellular morphology |
spherical
|
| Production |
melanin
|
| Growth Pattern |
suspension
|
| Histopathology |
malignant melanoma; from metastatic site
|
| D3S1358 |
14,16
|
|---|---|
| vWA |
16,17
|
| FGA |
18,20
|
| Amelogenin |
X,Y
|
| TH01 |
6
|
| TPOX |
11
|
| CSF1P0 |
12,13
|
| D5S818 |
12,13
|
| D13S317 |
11
|
| D7S820 |
12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium (Eagle) in Earle
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Cultures can be mainteined by addition or replacement of fresh medium. Establish new cultures at 1x10 exp5 cells/ml and maintain at 2x10 exp5.
|
| media change |
2 to 3 times per week
|
|
Oettgen HF et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J.Natl.Cancer Inst.Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One humdred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
