3T3-L1
| KCLB NO |
10092.1
|
|---|---|
| Name |
3T3-L1
|
세포주 분양조건
서비스
수량
-
+
| Origin |
embryo
|
|---|---|
| Species |
mouse mouse
|
| Reverse Transcriptase |
negative
|
| Cellular morphology |
fibroblast
|
| Production |
triglycerides
|
| Growth Pattern |
monolayer
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated bovine calf serum (BCS), 10%
|
| kclb media |
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated bovine calf serum (BCS), 10%
|
| note |
Cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation; tested and found negative for ectromelia virus (mousepox); the cells should be grown in plastic flasks, they do not grow well on some types of glass surfaces; a saturation density of approximately 50000 cells per sq cm can be reached.
|
|
Cell, 3:127, 1974
|
|---|
