세포주 정보

MDA-MB-231
MDA-MB-231
KCLB NO
30026
Name
MDA-MB-231
세포주 분양조건
서비스
수량
- +
Origin
breast, mammary gland
Species
human female, 51 years old, Caucasian
Receptor
epidermal growth factor(EGF), transforming growth factor alpha(TGF-alpha)
Tumorigenecity
Yes, in nude mice also in ALS treated BALB/c mice; forms poorly differentiated adenocarcinoma(grade III)
Isoenzyme
Me-2,1-2;PGM3,1;PGM1,1-2;ES-D,1;AK-1,1;GLO-1,2;G6PD B
Cellular morphology
epithelial
Growth Pattern
monolayer
Histopathology
adenocarcinoma; pleural effusion
D3S1358
16
vWA
15,18
FGA
22,23
Amelogenin
X
TH01
7,9.3
TPOX
8,9
CSF1P0
12,13
D5S818
12
D13S317
13
D7S820
8,9
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
Leibovitz
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove medium, rinse with fresh 0.25 % trypsin solution , remove trypsin and let the culture sit at room temperature (or at 37
Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980Siciliano MJ et al. Mutually exclusive genetic sihnatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979