SiHa
| KCLB NO |
30035
|
|---|---|
| Name |
SiHa
|
세포주 분양조건
서비스
수량
-
+
| Origin |
cervix, uterine
|
|---|---|
| Species |
human female, 55 years old, Mongoloid
|
| Tumorigenecity |
Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III)
|
| Isoenzyme |
PGM3,1-2;PGM1,1;ES-D,2;AK-1,1;Me-2,1;GLO-1,2;G6PD,B
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, squamous cell
|
| Oncogene |
pRB+; p53 +
|
| D3S1358 |
16,17
|
|---|---|
| vWA |
14,17
|
| FGA |
21
|
| Amelogenin |
X
|
| TH01 |
6,9
|
| TPOX |
8
|
| CSF1P0 |
12
|
| D5S818 |
9
|
| D13S317 |
11
|
| D7S820 |
10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids ad sodium pyruvate, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| subculturing |
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 min., remove trypsin, add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
| note |
This line is reported to contain an integrated human papillomavirus type 16 genome (HPV-16, 1 to 2 copies per cell).
|
|
Baker CC et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 16: 962-967, 1987Friedl F et al. Studies on a new human cell line (Siha) derived from carcinoma of uterus.I. Its establishment and morphology. Proc.Soc.Exp.Biol.Med. 135: 543-545, 1970
|
|---|
