CRFK
| KCLB NO |
10094
|
|---|---|
| Name |
CRFK
|
세포주 분양조건
서비스
수량
-
+
| Origin |
kidney, cortex
|
|---|---|
| Species |
cat
|
| VirusSusceptibility |
Feline herpes virus; feline calicivirus; feline panleukopenia; feline reovirus; canine parvovirus; rabiesvirus; BVD virus
|
| Reverse Transcriptase |
negative
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
normal
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium Eagle with 2mM L-glutamine and Earle's BSS adjusted to contain 1.5g/L sodium bicarbonate, 0.1 Mm non-essential amino acids, and 1.0 Mm sodium pyruvate 90%; horse serum 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to stand at room temperature ( or at 37''C)
until cells detach. Add fresh medium aspirate and dispense into new flasks.
|
| note |
The Cells are free of bovine viral diarrhea(BVD) virus.
|
|
Crandell RA et al. Development. Characterization, and viral susceptibilityof a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. Fabricant CG and Rich LJ. Microbial studies of feline urolithiasis. J. AM. Vet. Med. Assoc. 158: 976-980
|
|---|
