CMT-93
| KCLB NO |
10223
|
|---|---|
| Name |
CMT-93
|
세포주 분양조건
서비스
수량
-
+
| Origin |
rectum
|
|---|---|
| Species |
mouse male, 19 months old, Mus musculus
|
| Strain |
C57BL/icrf
|
| Reverse Transcriptase |
positive
|
| Tumorigenecity |
yes, in nude mice
|
| Cellular morphology |
epithelial
|
| Histopathology |
carcinoma, polyploid
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; heat inactivated fetal bovine serum (FBS), 10%- OR - bovine calf serum, 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| subculturing |
remove medium , add fresh 0.25% trypsin solution for 2 to 4minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes, add fresh medium, aspirate and dispense into new flask
|
| note |
Tested and found negative for ectromelia virus (mousepox)
|
|
Franks LM and Hemmings VJ. A cell line from an induced carcinoma of mouse rectum. J. Pathol. 124: 35-38, 1978.
|
|---|
