세포주 정보

RAG
RAG
KCLB NO
10142
Name
RAG
세포주 분양조건
서비스
수량
- +
Origin
kidney
Species
mouse Mus musculus
Strain
BALB/c
VirusSusceptibility
vesicular stomatitis (Indiana); herpes simplex
Virus Resistance
poliovirus 1
Reverse Transcriptase
positive
Cellular morphology
amoeboid
Production
kidney specific esterase-2 (ES-2)
Growth Pattern
monolayer
Histopathology
adenocarcinoma, renal
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
Eagle's Minimum Essential Medium in Earle's BBS with non-essential amino acids and 1mM sodium pyruvate, 9%; heat inactivated fetal bovine serum (FBS), 10%
kclb media
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
note
this is a non-reverting 8-azaguianine resistant (HPRT -, HGPRT -) clone of the Renal-2a cell line; tested and found negative for ectromelia virus (mousepox)
Science, 145:709, 1964; J Nat'l Cancer Inst, 43:319-333, 1969; J Cell Biol 45:74-82, 1970; Proc Nat'l Acad Sci USA 66:1220-1227, 1970