TM4
| KCLB NO |
21715
|
|---|---|
| Name |
TM4
|
세포주 분양조건
서비스
수량
-
+
| Origin |
testis; Sertoli cell
|
|---|---|
| Species |
mouse male, 11 to 13 days old
|
| Strain |
BALB/c nu/+
|
| Receptor |
follicle stimulating hormone (FSH); androgen; estrogen; progesterone
|
| Cellular morphology |
epithelial
|
| Histocompatibility |
H-Y
|
| Production |
transferrin; H-Y antigen; retinol binding protein; plasminogen activator
|
| Growth Pattern |
monolayer
|
| Histopathology |
normal
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's Medium with 4.5g/L glucose, 1.2g/L sodium bicarbonate and 15mM HEPES, 92.5%; horse serum, 5%; heat inactivated fetal bovine serum (FBS), 2.5%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| note |
the TM4 cell line responds to FSH with an increase in cAMP production, but does not respond to luteinizing hormone (LH); the FSH responsiveness is much reduced compared to primary Sertoli cell cultures; constitutive plasminogen activator production is low, but is stimulated by FSH and, to a greater extent, by retinoic acid; tested and found negative for ectromelia virus (mousepox)
|
|
Bio Repro, 23:243-250, 1980; Ann N.Y. Acad Sci, 383:44-68, 1982
|
|---|
