HCC-1359
| KCLB NO |
71359
|
|---|---|
| Name |
HCC-1359
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human female, 55 years old
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
spindle-giant cell tumor
|
| Depositor |
Gazdar AF
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Wistuba II et al., Comparison of features of human lung cancer cell lines and their corresponding tumors, Clin Cancer Res, 1999, 5:991-1000
|
|---|
