NCTC clone 1469; derivative of NCTC 721
| KCLB NO |
10009.1
|
|---|---|
| Name |
NCTC clone 1469; derivative of NCTC 721
|
세포주 분양조건
서비스
수량
-
+
| Origin |
liver
|
|---|---|
| Species |
mouse male, neonatal
|
| Strain |
C3H/An
|
| VirusSusceptibility |
vesicular stomatitis (Indiana)
|
| Virus Resistance |
poliovirus 1
|
| Reverse Transcriptase |
positive
|
| Cellular morphology |
epithelial
|
| Histocompatibility |
H-2k
|
| Growth Pattern |
monolayer
|
| Histopathology |
normal
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
NCTC 135 medium, 90%; horse serum, 10%
|
| kclb media |
Dulbecco's Modified Eagle's Medium, 90%; horse serum, 10%
|
| note |
the culture medium in heavy cultures may be turbid in appearance and give the gross impression of bacterial contamination. It is characteristic of the line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures; tested and found negative for ectromelia virus (mousepox)
Subcultures are prepared by scraping.
|
|
J Nat
|
|---|
