Malme-3M
| KCLB NO |
30064
|
|---|---|
| Name |
Malme-3M
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human male, 43 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice; forms pigmented malignant melanoma
|
| Isoenzyme |
Me-2,1;PGM3,1;PGM1,1;ES-D,1;AK-1,1;GLO-1,1-2;G6PD,B
|
| Karyology |
(P2) hypertriploid to hypotetraploid with large submetacentric marker
|
| Cellular morphology |
mixed
|
| Growth Pattern |
monolayer
|
| Histopathology |
malignant melanoma; from metastatic site
|
| D3S1358 |
14,18
|
|---|---|
| vWA |
15,16
|
| FGA |
21,22
|
| Amelogenin |
X,Y
|
| TH01 |
8
|
| TPOX |
8,9
|
| CSF1P0 |
12
|
| D5S818 |
11
|
| D13S317 |
8,13
|
| D7S820 |
9,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Leibovitz's L-15 medium, 85%; heat inactivated fetal bovine serum (FBS), 15%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach (about 10min).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
|
| splitratio |
1:2 to 1:4
|
| media change |
Twice per week
|
|
Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One humdred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
