SK-HEP-1
| KCLB NO |
30052
|
|---|---|
| Name |
SK-HEP-1
|
세포주 분양조건
서비스
수량
-
+
| Origin |
liver
|
|---|---|
| Species |
human male, 52 years old, causasian
|
| Tumorigenecity |
Yes, in nude mice; forms large cell carcinoma consistent with hepatoma
|
| Isoenzyme |
Me-2,2;PGM3,1-2;PGM1,1;ES-D,1-2;AK-1,1;GLO-1,1-2;G6PD,B
|
| Karyology |
(P11) hyperdiploid to hypotriploid (+A3,+C,+E,+F,+G,-A,-D) with abnormalities
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma; ascites
|
| D3S1358 |
16
|
|---|---|
| vWA |
14,17
|
| FGA |
17
|
| Amelogenin |
X
|
| TH01 |
7,9
|
| TPOX |
9
|
| CSF1P0 |
11,12
|
| D5S818 |
10,13
|
| D13S317 |
8,12
|
| D7S820 |
8,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium (Eagle) in Earle
|
| kclb media |
Dulbecco
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
| splitratio |
1:2 to 1:4
|
| media change |
2 to 3 times weekly
|
|
Turner BM and Turner VS. Secretion of alpha1-antitrypsin by an established human hepatoma cell line and by human/mouse hybrids. Somatic Cell Genet. 6:1-14, 1980. Fogh J et al. Absence of Hela cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cacer Inst. 59: 221-226, 1977
|
|---|
