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SNU-16

KCLB NO	00016
Name	SNU-16

세포주 분양조건

비영리 동결(Frozen)
비영리 배양(Culture)
영리 동결(Frozen)
영리 배양(Culture)

서비스

DNA extraction service
cDNA synthesis service
Pellet service

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Origin	stomach; ascites
Species	human, asian female, 33 years old
Abnormal genes	p53: codon 205, TAT to TTT, Tyr to Phe
Cellular morphology	lymphoblast-like
Growth Pattern	suspension
Histopathology	adenocarcinoma
Differentiation	poorly differentiated
Depositor	Jae-Gahb Park, M.D.
DateOfUpdated	2014-09-11
Option	culture or frozen

D3S1358	15,16
vWA	16
FGA	23
Amelogenin	X
TH01	9
TPOX	11
CSF1P0	12
D5S818	10,13
D13S317	8,12
D7S820	12

준비중입니다.

freeze media	RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing	Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
populaton doubling time	27 hours
splitratio	1:4
media change	Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days

Cancer Res, 50: 2773-2780, 1990; J Nat
Shin KH, Yang YM, Park JG: Absence or decreased levels of the hMLH1 protein in human gastric carcinoma cell lines: implication of hMLH1 in alkylation tolerance. J Cancer Res Clin Oncol 124 8 421-6 ▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 00216, 00484, 00520, 00601, 00620, 00638, 00668, 00719 ] Kang MS, Kim HS, Han JA, Park SC, Kim WB, Park JG: Characteristics of human gastric carcinoma cell lines with induced multidrug resistance. Anticancer Res 17 5A 3531-6 Sep 1997 ▶ ref. kclb cell line no.'s - [ 00001, 00016 ] Kim DK, Kim HT, Tai JH, Cho YB, Kim TS, Kim KH, Park JG, Hong WS: Pharmacokinetics and antitumor activity of a new platinum compound, cis-malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1, 3- dioxolane]platinum(II), as determined by ex vivo pharmacodynamics. Cancer Chemother Pharmacol 37 1-2 1-6 1995 ▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 80103, 30103, {PC-9}, {PC-14} ] Bae CD, Park SE, Seong YS, Kimm SW, Park JB, Park JB: The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines. J Korean Med Sci 8 2 153-9 Apr 1993 ▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 30103 ] Choe G, Kim WH, Park JG, Kim YI: Effect of suramin on differentiation of human stomach cancer cell lines. J Korean Med Sci 12 5 433-42 Oct 1997 ▶ ref. kclb cell line no.'s - [ 00005, 00016 ] Ahn CM, Kim SK, Han JL: Synthesis of 6-aziridinylbenzimidazole derivatives and their in vitro antitumor activities. Arch Pharm Res 21 5 599-609 Oct 1998 ▶ ref. kclb cell line no.'s - [ 00016, {B16}, {P388} ] Park YS, Kim YH, Kim SK, Choi SJ: A new antitumor agent: methyl sulfonium perchlorate of echinomycin. Bioorg Med Chem Lett 7 8(7) 731-4 Apr 1998 ▶ ref. kclb cell line no.'s - [ 00016, {B16}, {P388} ] Hara T, Ooi A, Kobayashi M, Mai M, Yanagihara K, Nakanishi I: Amplification of c-myc, K-sam, and c-met in gastric cancers: detection by fluorescence in situ hybridization. Lab Invest 78 9 1143-53 Sep 1998 ▶ ref. kclb cell line no.'s - [ 00016, 30103, {HSC-39} ] Mor O, Ranzani GN, Ravia Y, Rotman G, Gutman M, Manor A, Amadori D, Houldsworth J, Hollstein M, Schwab M, et al: DNA amplification in human gastric carcinomas. Cancer Genet Cytogenet 65 2 111-4 Feb 1993 ▶ ref. kclb cell line no.'s - [ 00016, 30103 ] Bar-Am I, Mor O, Yeger H, Shiloh Y, Avivi L: Detection of amplified DNA sequences in human tumor cell lines by fluorescence in situ hybridization. Genes Chromosomes Cancer 4 4 314-20 Jun 1992 ▶ ref. kclb cell line no.'s - [ 00016, 30103, {NUB-7}, {NUB-20} ] Park JG, Frucht H, LaRocca RV, Bliss DP Jr, Kurita Y, Chen TR, Henslee JG, Trepel JB, Jensen RT, Johnson BE, et al: Characteristics of cell lines established from human gastric carcinoma. Cancer Res 1 50(9) 2773-80 May 1990 ▶ ref. kclb cell line no.'s - [ 00016 ] Park BK, Moon HR, Yu JR, Kook J, Chai JY, Lee SH: Comparative susceptibility of different cell lines for culture of Toxoplasma gondii in vitro. Korean J Parasitol 31 3 215-22 Sep 1993 ▶ ref. kclb cell line no.'s - [ 00001, 00016, 10034, 10081, 10185, 10240, 80103, {PC-14} ]

Cancer Res, 50: 2773-2780, 1990; J Nat

Shin KH, Yang YM, Park JG: Absence or decreased levels of the hMLH1 protein in human gastric carcinoma cell lines: implication of hMLH1 in alkylation tolerance. J Cancer Res Clin Oncol 124 8 421-6
▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 00216, 00484, 00520, 00601, 00620, 00638, 00668, 00719 ]
Kang MS, Kim HS, Han JA, Park SC, Kim WB, Park JG: Characteristics of human gastric carcinoma cell lines with induced multidrug resistance. Anticancer Res 17 5A 3531-6 Sep 1997
▶ ref. kclb cell line no.'s - [ 00001, 00016 ]
Kim DK, Kim HT, Tai JH, Cho YB, Kim TS, Kim KH, Park JG, Hong WS: Pharmacokinetics and antitumor activity of a new platinum compound, cis-malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1, 3- dioxolane]platinum(II), as determined by ex vivo pharmacodynamics. Cancer Chemother Pharmacol 37 1-2 1-6 1995
▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 80103, 30103, {PC-9}, {PC-14} ]
Bae CD, Park SE, Seong YS, Kimm SW, Park JB, Park JB: The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines. J Korean Med Sci 8 2 153-9 Apr 1993
▶ ref. kclb cell line no.'s - [ 00001, 00005, 00016, 30103 ]
Choe G, Kim WH, Park JG, Kim YI: Effect of suramin on differentiation of human stomach cancer cell lines. J Korean Med Sci 12 5 433-42 Oct 1997
▶ ref. kclb cell line no.'s - [ 00005, 00016 ]
Ahn CM, Kim SK, Han JL: Synthesis of 6-aziridinylbenzimidazole derivatives and their in vitro antitumor activities. Arch Pharm Res 21 5 599-609 Oct 1998
▶ ref. kclb cell line no.'s - [ 00016, {B16}, {P388} ]
Park YS, Kim YH, Kim SK, Choi SJ: A new antitumor agent: methyl sulfonium perchlorate of echinomycin. Bioorg Med Chem Lett 7 8(7) 731-4 Apr 1998
▶ ref. kclb cell line no.'s - [ 00016, {B16}, {P388} ]
Hara T, Ooi A, Kobayashi M, Mai M, Yanagihara K, Nakanishi I: Amplification of c-myc, K-sam, and c-met in gastric cancers: detection by fluorescence in situ hybridization. Lab Invest 78 9 1143-53 Sep 1998
▶ ref. kclb cell line no.'s - [ 00016, 30103, {HSC-39} ]
Mor O, Ranzani GN, Ravia Y, Rotman G, Gutman M, Manor A, Amadori D, Houldsworth J, Hollstein M, Schwab M, et al: DNA amplification in human gastric carcinomas. Cancer Genet Cytogenet 65 2 111-4 Feb 1993
▶ ref. kclb cell line no.'s - [ 00016, 30103 ]
Bar-Am I, Mor O, Yeger H, Shiloh Y, Avivi L: Detection of amplified DNA sequences in human tumor cell lines by fluorescence in situ hybridization. Genes Chromosomes Cancer 4 4 314-20 Jun 1992
▶ ref. kclb cell line no.'s - [ 00016, 30103, {NUB-7}, {NUB-20} ]
Park JG, Frucht H, LaRocca RV, Bliss DP Jr, Kurita Y, Chen TR, Henslee JG, Trepel JB, Jensen RT, Johnson BE, et al: Characteristics of cell lines established from human gastric carcinoma. Cancer Res 1 50(9) 2773-80 May 1990
▶ ref. kclb cell line no.'s - [ 00016 ]
Park BK, Moon HR, Yu JR, Kook J, Chai JY, Lee SH: Comparative susceptibility of different cell lines for culture of Toxoplasma gondii in vitro. Korean J Parasitol 31 3 215-22 Sep 1993
▶ ref. kclb cell line no.'s - [ 00001, 00016, 10034, 10081, 10185, 10240, 80103, {PC-14} ]

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