SNU-4116
| KCLB NO |
04116
|
|---|---|
| Name |
SNU-4116
|
세포주 분양조건
서비스
수량
-
+
| Origin |
Glioblastoma*
|
|---|---|
| Species |
human, asian female, 57 years old
|
| Growth Pattern |
Monolayer
|
| Option |
culture only
|
| D3S1358 |
15
|
|---|---|
| vWA |
17,19
|
| FGA |
22,24
|
| Amelogenin |
X
|
| TH01 |
7,9
|
| TPOX |
8,11
|
| CSF1P0 |
10,11
|
| D5S818 |
9,11
|
| D13S317 |
13,14
|
| D7S820 |
11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
OPTI-MEM-I , inactivated fetal bovine serum (FBS)5%
|
| kclb media |
OPTI-MEM-I , inactivated fetal bovine serum (FBS)5%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask.
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Scientific data vol. 10,1 448. 12 Jul. 2023
|
|---|
