세포주 정보

NCI-H82
NCI-H82
KCLB NO
30175
Name
NCI-H82
세포주 분양조건
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Origin
lung; pleural effusion
Species
human human
Tumorigenecity
yes; forms transplantable tumors with non-typical SCLC histology
Isoenzyme
G6PD, B; PGM1, 1-2; PGM3, 1-2; ES-D, 1; Me-2, 1; AK-1; GLO-1, 1
Karyology
This is a near triploid human cell line. The modal chromosome number is 68, occurring at 44% with polyploidy at 3%. Each cell had two copies of a normal X chromosome. The Y chromosome was not detected in Q banded preparations
Cellular morphology
epithelial
Growth Pattern
aggregates in suspension; the cells grow in very large aggregates, and the aggregates are the only viable cell population
Histopathology
small cell lung cancer (SCLC)
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%